Whole Genome Sequence of Strix owl - SRA

SRX3182907: Whole Genome Sequence of Strix owl
1 ILLUMINA (Illumina HiSeq 2500) run: 9.1M spots, 1.8G bases, 997.4Mb downloads

Design: We used 10 ng genomic DNA to prepare a whole-genome library using a Nextera DNA Sample Preparation Kit (Illumina). After tagmentation, we cleaned the reaction with a DNA Clean & Concentrator -5 kit (Zymo Research). We amplified the reaction and added Illumina indexed adapters with five cycles of PCR using a KAPA Library Amplification kit (KAPA Biosystems) and then cleaned the reaction with a DNA Clean & Concentrator -5 kit (Zymo Research). We assessed the concentration of double-stranded DNA material with a Qubit 2.0 Flurometer (Invitrogen), combined the library into an equimolar sixteen-sample pool, and then concentrated the pool with a DNA Clean & Concentrator -5 kit (Zymo Research). We used a BluePippin (Sage Science) to select library fragments in the size range of 550-750 nt, which, after subtracting the 134 nt of adapters, corresponded to selecting an average insert size of 516 nt. We then performed a real-time PCR (rtPCR) using a KAPA Real-Time Library Amplification Kit (KAPA Biosystems) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) to amplify the pool with nine cycles of PCR. We then cleaned the PCR products with a DNA Clean & Concentrator -5 kit (Zymo Research). We assessed the concentration of double-stranded DNA material with a Qubit 2.0 Flurometer (Invitrogen) and the library fragment size distribution with a 2100 BioAnalyzer (Agilent Technologies), which revealed that the average fragment size of the pool was 607 nt. We combined this pool in an equimolar ratio with another library and then sequenced this new pool across both lanes of a two-lane flow cell with a HiSeq PE Rapid Cluster Kit and a 200 cycle HiSeq Rapid SBS Kit v1 on a HiSeq 2500 (Illumina).

Submitted by: University of California, Berkeley

Study: Genome sequencing of Spotted and Barred Owls

PRJNA406821 • SRP117279 • All experiments • All runs

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We performed whole genome sequencing of Spotted (<i>Strix occidentalis</i>) and Barred Owls (<i>Strix varia</i>) in order to study populations in western North America.

Sample: CU51478

SAMN07637302 • SRS2511471 • All experiments • All runs

Organism: Strix varia varia

Library:

Name: CU51478_run1_lane1

Instrument: Illumina HiSeq 2500

Strategy: WGS

Source: GENOMIC

Selection: Restriction Digest

Layout: PAIRED

Runs: 1 run, 9.1M spots, 1.8G bases, 997.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR6032936	9,063,037	1.8G	997.4Mb	2019-03-25

ID:: 4484860

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